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Opentrons is a laboratory automation application developed by Opentrons Labworks that enables researchers to design, simulate, and execute liquid-handling protocols on the company’s OT-2 and Flex robotic platforms. Currently at version 9.0.0 and offered in two distinct release branches, the program provides a visual protocol designer for drag-and-step workflow construction, a built-in Python API for scripting complex experiments, and real-time run monitoring that displays pipette position, labware status, and progress timestamps. Typical use cases include high-throughput plating, serial dilution, PCR and NGS library preparation, magnetic-bead clean-ups, and automated sample aliquoting in academic, clinical, and biotech settings. The software automatically generates calibration routines for tips, tubes, and plates, integrates with the Opentrons module ecosystem for temperature, magnetic, and shaking control, and exports run logs in CSV or JSON formats for LIMS compliance. Because the application is the sole control interface for Opentrons hardware, it functions simultaneously as an IDE for protocol development, a simulator for validating liquid-level and timing parameters, and a runtime dashboard that can pause, resume, or abort jobs without compromising sterility. Category placement falls under Science / Laboratory Automation, reflecting its specialized role in life-science workflows. Opentrons is available for free on get.nero.com, with downloads supplied through trusted Windows package sources such as winget, always delivering the latest version and supporting batch installation of multiple applications.
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